sirt1 elisa kit Search Results


sirt1 elisa kit  (Bioss)
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Bioss sirt1 elisa kit
Sirt1 Elisa Kit, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sirt1 (sirtuin 1) elisa kit  (Guangzhou JET Bio-Filtration)
93
Guangzhou JET Bio-Filtration human sirt1 (sirtuin 1) elisa kit
Human Sirt1 (Sirtuin 1) Elisa Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tnf α concentrations  (Cusabio)
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Cusabio tnf α concentrations
Tnf α Concentrations, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirt1  (Cusabio)
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Cusabio sirt1
CR and the <t>AMPK-SIRT1-mTOR</t> network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase <t>Sirtuin-1</t> (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.
Sirt1, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nad dependent deacetylase sirtuin 1 sirt1 sir2l1 elisa kits  (Cusabio)
93
Cusabio human nad dependent deacetylase sirtuin 1 sirt1 sir2l1 elisa kits
CR and the <t>AMPK-SIRT1-mTOR</t> network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase <t>Sirtuin-1</t> (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.
Human Nad Dependent Deacetylase Sirtuin 1 Sirt1 Sir2l1 Elisa Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits for sirt1  (AFG Bioscience LLC)
90
AFG Bioscience LLC elisa kits for sirt1
CR and the <t>AMPK-SIRT1-mTOR</t> network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase <t>Sirtuin-1</t> (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.
Elisa Kits For Sirt1, supplied by AFG Bioscience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirtuin-1 (sirt1) kits  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology sirtuin-1 (sirt1) kits
CR and the <t>AMPK-SIRT1-mTOR</t> network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase <t>Sirtuin-1</t> (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.
Sirtuin 1 (Sirt1) Kits, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sirtuin 1  (Absolute Biotech Inc)
90
Absolute Biotech Inc human sirtuin 1
Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , <t>SIRT1</t> (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.
Human Sirtuin 1, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirt1 elisa kit  (Elabscience Biotechnology)
90
Elabscience Biotechnology sirt1 elisa kit
Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , <t>SIRT1</t> (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.
Sirt1 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human nad-dependent deacetylase sirtuin-1 sirt1 elisa kit  (EIAab Inc)
90
EIAab Inc human nad-dependent deacetylase sirtuin-1 sirt1 elisa kit
Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , <t>SIRT1</t> (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.
Human Nad Dependent Deacetylase Sirtuin 1 Sirt1 Elisa Kit, supplied by EIAab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sirtuin 1 (sirt1) elisa kit, cat. no. sg-10458  (SinoGeneclon Biotech Co Ltd)
90
SinoGeneclon Biotech Co Ltd human sirtuin 1 (sirt1) elisa kit, cat. no. sg-10458
Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , <t>SIRT1</t> (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.
Human Sirtuin 1 (Sirt1) Elisa Kit, Cat. No. Sg 10458, supplied by SinoGeneclon Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-sirt1  (Signalway Antibody)
90
Signalway Antibody anti-sirt1
Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , <t>SIRT1</t> (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.
Anti Sirt1, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CR and the AMPK-SIRT1-mTOR network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase Sirtuin-1 (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.

Journal: Aging (Albany NY)

Article Title: The consequences of a high-calorie diet background before calorie restriction on skeletal muscles in a mouse model

doi: 10.18632/aging.203237

Figure Lengend Snippet: CR and the AMPK-SIRT1-mTOR network. AMPKs were analyzed by qPCR (mRNA levels) and Western Blot (protein levels) in pectoralis major (PM) ( A , B ) and quadriceps femoris vastus medialis ( QF - VM ) ( C , D ). SIRT1 was analyzed in PM and QF- VM ( E ) with the Mouse NAD-Dependent Deacetylase Sirtuin-1 (SIRT1) ELISA Kit (CUSABIO) for quantitative determination, according to the assay kit protocol . Values are expressed in ng/ml. mTOR mRNA in PM and QF- VM ( F ). Immunoblot results and protein expression of mTOR ( G ) and mTOR-S2448 ( H ) in PM and QF- VM . For the qPCR assay, each primer was analyzed with SYBR Green fluorescence detection and the transcript levels, expressed as a %, were normalized to those of the endogenous control 18s rRNA. Protein expressions were obtained by Western Blot analysis and quantified with Image Lab 6.1 software. SD expression was set as 1 and the relative protein levels were normalized as a ratio of GAPDH expression. The data are the mean ± s.d. *P < 0.05; **P < 0.01 vs SD, unless otherwise specified.

Article Snippet: One hundred mg of PM and QF- VM tissue homogenates from the SD (n=10), SD-CR (n=19), HC (n=14), and HC-CR (n=33) were analyzed with a Mouse NAD-Dependent Deacetylase Sirtuin-1 (SIRT1) ELISA Kit (CUSABIO) for the quantitative determination of SIRT1, according to its manual instructions (Supplementary Material 3).

Techniques: Western Blot, Histone Deacetylase Assay, Enzyme-linked Immunosorbent Assay, Expressing, SYBR Green Assay, Fluorescence, Control, Software

Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , SIRT1 (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.

Journal: Frontiers in Immunology

Article Title: Epigenetically altered macrophages promote development of diabetes-associated atherosclerosis

doi: 10.3389/fimmu.2023.1196704

Figure Lengend Snippet: Plaque macrophages in diabetic patients express significantly high HDAC3. (A) Representative images for LCM technique to extract either CD31+ endothelial cells or CD68+ macrophages within atherosclerotic lesions of human patients. (B) Serum HbA1C percentages in non-diabetic and diabetic groups. (C–G) ELISA for HDAC3 (C) , EZH2 (D) , DNMT1 (E) , SIRT1 (F) , and JMJD3 (G) in CD31+ endothelial cells and CD68+ macrophages from the plaques of both non-diabetic and diabetic cases. *p<0.05. Scale bars were 50µm.

Article Snippet: ELISA for human HDAC3 (LS-F55924, LSBio, Seattle, WA, USA), human enhancer of zeste homolog 2 (EZH2; LS-F7278, LSBio), human DNA Methyltransferase 1 (DNMT1; LS-F7340, LSBio), human sirtuin 1 (SIRT1; LS-F12606, LSBio), human jumonji domain containing 3 (JMJD3; LS-F74347, LSBio), mouse IL-6 (Ab100713, Abcam), mouse IL-1β (Ab197742, Abcam), mouse tumor necrosis factor alpha (TNFα; Ab208348, Abcam), mouse interferon gamma (IFNγ; Ab282874, Abcam), mouse arginase 1 (ARG1; Ab269541, Abcam), mouse CD163 (Ab272204, Abcam), mouse α-smooth muscle actin (α-SMA; NBP2-66429, Novus Biologicals, Shanghai, China), Vimentin (LS-F7624, LSBio, Seattle, WA, USA) and mouse Collagen IV (LS-F20750, LSBio) were utilized as per the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay